Benefits

• Promotes healthy digestion and bowel regularity
• Helps restore and maintain gut microbial balance
• Supports immune health and inflammatory balance
• Enhances intestinal barrier function
• May benefit mood through the gut-brain connection

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What’s Inside

5 Spore-Based Probiotic Strains

Formulated with over 10 billion CFU of clinically studied Bacillus strains:

• Bacillus subtilis DE111® (5 billion CFU): Promotes immune health, gut microbial balance, and SCFA production.
• Bacillus coagulans (LactoSpore®) (2 billion CFU): Supports digestion, immune regulation, and relief from IBS symptoms.
• Bacillus coagulans (SNZ1969) (1 billion CFU): Enhances intestinal regularity and digestive comfort.
• Bacillus coagulans (Lacris-15) (100 million CFU): Contributes to microbial balance and GI function.
• Bacillus clausii (CSI-08) (2 billion CFU): Offers antimicrobial support and modulates inflammation.

Spore-Forming Advantage

Unlike lactobacillus strains, these hardy probiotics are encapsulated in protective spores, allowing them to survive harsh conditions like stomach acid and heat. They become active once they reach the intestines—where they do their job best.

No Refrigeration Needed

Resilient and shelf-stable, these probiotics don’t require refrigeration and maintain potency in a variety of environments.

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How to Use

Take 1 capsule daily with a meal or as directed by your health-care practitioner.

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What to Avoid

Consult your health-care provider before use if you are pregnant, nursing, or taking medications.

Do not exceed recommended dose.

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

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Purity and Safety

Free from gluten, dairy, soy, and GMOs. No refrigeration required.

Formulated with clinically studied, evidence-backed ingredients.

DE111® and LactoSpore® are registered trademarks of their respective companies.

Research:
1. Elshaghabee FMF, Rokana N, Gulhane RD, Sharma C, Panwar H. Bacillus as potential probiotics: status, concerns, and future perspectives. Front Microbiol. 2017;8:1490. doi:10.3389/fmicb.2017.01490
2. Mazhar S, Khokhlova E, Colom J, Simon A, Deaton J, Rea K. In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111®. Front Microbiol. 2022;13:1101144. doi:10.3389/fmicb.2022.1101144
3. Colom J, Freitas D, Simon A, et al. Acute physiological effects following Bacillus subtilis DE111 oral ingestion – a randomised, double blinded, placebo-controlled study. Benef Microbes. 2023;14(1):31-43. doi:10.3920/BM2022.0081
4. Colom J, Freitas D, Simon A, et al. Presence and germination of the probiotic Bacillus subtilis DE111® in the human small intestinal tract: a randomized, crossover, double-blind, and placebo-controlled study. Front Microbiol. 2021;12:715863. doi:10.3389/fmicb.2021.715863
5. Wong JMW, de Souza R, Kendall CWC, Emam A, Jenkins DJA. Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol. 2006;40(3):235-243. doi:10.1097/00004836-200603000-00015
6. DE111® Bacillus subtilis. DE111 survivability in simulated in gastric and intestinal fluid. Deerland Probiotics and Enzymes; 2017
7. Shinde T, Vemuri R, Shastri MD, et al. Probiotic Bacillus coagulans MTCC 5856 spores exhibit excellent in-vitro functional efficacy in simulated gastric survival, mucosal adhesion and immunomodulation. J Funct Foods. 2019;52:100-108.doi:10.1016/j.jff.2018.10.031
8. Elisashvili V, Kachlishvili E, Chikindas ML. Recent advances in the physiology of spore formation for Bacillus probiotic production. Probiotics Antimicrob Proteins. 2019;11(3):731-747. doi:10.1007/s12602-018-9492-x
9. Tang R, Li L. Modulation of short-chain fatty acids as potential therapy method for type 2 diabetes mellitus. Can J Infect Dis Med Microbiol J Can Mal Infect Microbiol Médicale. 2021;2021:6632266. doi:10.1155/2021/6632266
10. Portincasa P, Bonfrate L, Vacca M, et al. Gut microbiota and short chain fatty acids: implications in glucose homeostasis. Int J Mol Sci. 2022;23(3):1105. doi:10.3390/ijms23031105
11. Zhang HL, Li WS, Xu DN, et al. Mucosa-reparing and microbiota-balancing therapeutic effect of Bacillus subtilis alleviates dextrate sulfate sodium-induced ulcerative colitis in mice. Exp Ther Med. 2016;12(4):2554-2562.doi:10.3892/etm.2016.3686
12. Ilinskaya ON, Ulyanova VV, Yarullina DR, Gataullin IG. Secretome of intestinal bacilli: a natural guard against pathologies. Front Microbiol. 2017;8:1666. doi:10.3389/fmicb.2017.01666
13. Ripert G, Racedo SM, Elie AM, Jacquot C, Bressollier P, Urdaci MC. Secreted compounds of the probiotic Bacillus clausii strain O/C inhibit the cytotoxic effects induced by Clostridium difficile and Bacillus cereus toxins. Antimicrob Agents Chemother. 2016;60(6):3445-3454. doi:10.1128/AAC.02815-15
14. Labellarte G, Maher M. Tolerance and effect of a probiotic supplement delivered in capsule form. Food Nutr Sci.2019;10(06):626-634. doi:10.4236/fns.2019.106046
15. Cuentas AM, Deaton J, Khan S, Davidson J, Ardita C. The effect of Bacillus subtilis DE111 on the daily bowel movement profile for people with occasional gastrointestinal irregularity. J Probiotics Health. 2017;05(04).doi:10.4172/2329-8901.1000189
16. Majeed M, Nagabhushanam K, Natarajan S, et al. Bacillus coagulans MTCC 5856 supplementation in the management of diarrhea predominant irritable bowel syndrome: a double blind randomized placebo controlled pilot clinical study. Nutr J. 2015;15(1):21. doi:10.1186/s12937-016-0140-6
17. Majeed M, Nagabhushanam K, Arumugam S, Majeed S, Ali F. Bacillus coagulans MTCC 5856 for the management of major depression with irritable bowel syndrome: a randomised, double-blind, placebo controlled, multi-centre, pilot clinical study. Food Nutr Res. 2018;62(0). doi:10.29219/fnr.v62.1218
18. Abhari K, Shekarforoush SS, Hosseinzadeh S, Nazifi S, Sajedianfard J, Eskandari
MH. The effects of orally administered Bacillus coagulans and inulin on prevention and progression of rheumatoid arthritis in rats. Food Nutr Res. 2016;60(1):30876. doi:10.3402/fnr.v60.30876
19. Freedman KE, Hill JL, Wei Y, et al. Examining the gastrointestinal and immunomodulatory effects of the novel probiotic Bacillus subtilis DE111. Int J Mol Sci. 2021;22(5):2453. doi:10.3390/ijms22052453
20. Rhayat L, Maresca M, Nicoletti C, et al. Effect of Bacillus subtilis strains on intestinal barrier function and inflammatory response. Front Immunol. 2019;10:564. doi:10.3389/fimmu.2019.00564
21. Townsend JR, Bender D, Vantrease WC, et al. Effects of probiotic (Bacillus subtilis DE111) supplementation on immune function, hormonal status, and physical performance in Division I baseball players. Sports Basel Switz. 2018;6(3):70. doi:10.3390/sports6030070